The key laboratory of the Ministry of Education is an important public platform for scientific research experiments in universities, and its construction and management is an effective means to promote the opening and sharing of scientific research resources. Take the Key Laboratory of New Technology for Construction of Cities in Mountain Area for example, combined with practical problems, this paper introduces five main experiences in laboratory construction:rationalization of talent echelon construction by introducing leading talents; enhancement of research security by increasing hardware investment; integration of industry, education, research and application to promote the development of science and education platform; expanding academic exchanges with foreign countries based on the characteristics of mountainous areas; managing virtual and reality alternation and realizing hardware environment usage to be shared. It can provide reference for operation and construction management of public platform for university scientific research experiments.