This article aims at the present situation of the increasingly serious humanistic spirit loss and absence of college students majoring in science and engineering. It analyzes the reasons why Chinese college students majoring in science and engineering generally lack humanistic quality, and puts forward concrete measures for strengthening the education of humanistic quality according to the present situation. With the hope of developing college students’ scientific education, this article highly emphasizes the cultivation of college students’ humanistic spirit as well. In addition, this article also proposes measures such as building campus culture environment with strong humanistic atmosphere, paying attention to students’ subjectivity in humanistic spirit construction, increasing humane landscapes on campus, adjusting specialty structure and course design, and energetically developing colorful extracurricular activities etc., in order to improve the effectiveness of humanistic spirit education at college and provide theoretical and practical references and strategies for the higher education reform and the cultivation of college students’ comprehensive quality in the 21st century.