To realize the objective of training application-oriented college students, we studied a professional course system of civil engineering, which well integrated theoretical teaching and practical teaching. A practical teaching system of civil engineering was constructed by a teaching platform consisting of basic experiment, professional practice and engineering skill training, and training of innovation and entrepreneurship. To ensure the performance quality and continuity of practical teaching system, guarantee measures and a ministering system were also analyzed. It is very important to develop a new practical teaching system which is suitable for and can promote discipline of civil engineering in technique-application-oriented universities.