Through the analysis of current situation and existing problems of university-industry cooperation practice teaching in civil engineering, the factors of the operating mechanism and the difference between them were expounded. The university-industry cooperation practice teaching mode under current civil engineering practice teaching mode and enterprise operation mode and the construction methods of its operating mechanism were proposed. The operating mechanism of industry-university-research cooperation practice teaching were designed and explored in the aspects of constructing organization and management department, modular teaching, building quality evaluation system and the solutions to relevant problems. Finally the recommendatory strategies were provided for the practice teaching of civil engineering.