The essence of innovation and entrepreneurship education is to cultivate innovative spirit and entrepreneurial ability of students, which requires higher education to transform and expand the personnel training mode. This paper took civil engineering professional talents training in Jiangnan University as an example, from the aspects of training plan and operation mechanism, reform of teaching content and method, and discipline innovation and entrepreneurship training platform construction, to conduct an in-depth analysis. An innovative education system consisting of mechanism, method and platform was constructed, to lay a good foundation for cultivating innovative and entrepreneurial talents in the field of civil engineering.