Space is the carrier of ecological civilization construction and territorial space planning is a key measure to promote ecological civilization construction. In the process of integrating urban and rural planning into territorial space planning, the concept, focus and technical methods of planning preparation have also undergone corresponding changes. In order to better implement Xi Jinping Thought on Ecological Civilization in the teaching of territorial spatial master planning course and solve the problems and difficulties in the teaching transformation in response to the development requirements of the new era, many architecture colleges have been actively exploring and practicing. This paper takes the undergraduate course of territorial spatial master planning of Kunming University of Science and Technology as an example to explore the specific measures of teaching reform, including the guidance of Xi Jinping Thought on Ecological Civilization, the expansion of knowledge structure of the intersection of ecology and urban and rural planning, and the application of GIS and other technologies. Finally, the teaching reform practice is demonstrated from three aspects: evaluation of the carrying capacity of resources and environment and the suitability evaluation of land and space development, the delimitation of the three zones and three lines, and the blue-green space planning of the central urban area, to provide a practical reference path for the teaching of territorial spatial master planning in the urban and rural planning specialty.