The theoretical essence of "harmony culture" which emphasizes order, harmony but "harmony but different" has great reference function for the creative transformation and innovative development of Chinese excellent traditional culture. As an important carrier of moral education in Colleges and universities, the practice base of moral education shoulders an important mission in the process of talent training in Colleges and universities. This paper intends to focus on the new requirements of the new era to solve the fragmentation and randomization problems of the second classroom practice course, to eliminate the difficulties of insufficient joint efforts and waste of resources in the construction of the practice base of moral education, to explore the construction of the "three one one " mode under the "harmony" perspective, to reconstruct the teaching staff, to start the construction of the database, and to improve the practice base of the second classroom moral education in Colleges and universities through the construction of the innovation platform In order to enhance the scientific and long-term effectiveness of the practice base system.