The Rural Revitalization and the construction of Guangdong-Hong Kong-Marco Greater Bay Area provide new opportunities for the development of civil engineering professionals in agricultural universities. In order to realize the goal of integrative talents training of civil engineering specialty in agricultural universities, which is "the knowledge of managerial,design and construction”, we should integrate the teaching resources inside and outside the school, Following the school-running characteristics of "work-based, combination of industry and agriculture", a “Five-in-One” talent training mode is created to reflect the characteristics of agricultural universities. The curriculum system,experimental teaching system, science and technology team of student, teaching staff , teaching quality monitoring and guarantee system have been constructed. Practice shows that the “Five-in-One” talent training model has achieved good results in terms of teaching conditions, teaching and research level of teachers, students’ comprehensive ability, demonstration and promotion of construction results and social impact.