In order to cultivate their devotion to family and country together with improving the frontier knowledge and their innovation ability, the postgraduate training mode of "Cultural Coupling" was defined under the background of Emerging Engineering Education (3E). And then, three platforms and the discipline culture were expounded. These platforms included the characteristic course system, good educational resources and practice terraces on multi-mode of research. After that, the cultural coupling mechanism was analyzed by three coupling points which were teaching activities, academic activities and the academic idol. Finally, through the exploration of "cultural coupling" postgraduate training mode in the discipline of transportation engineering at Southeast University, the result turned out is remarkably effective. It can provide reference to use for implementing of fostering virtue through education and improving the quality of postgraduate training.