To meet the dual demands of talent supply and technological innovation in the high-quality development of the civil engineering sector, a party organization collaboration mechanism between Civil Engineering-focused Universities and Industry Chains serves as a key driver for integrating education, science, and talent development. This study analyzes typical bottlenecks in university-enterprise cooperation, including insufficient precision in alignment, weak continuity in interaction, uncoordinated operational mechanisms, and incomplete systemic support. In response, a four-dimensional mechanism of strengthening-supplementing-extending-consolidating is developed. The mechanism includes innovations in supply-demand matching, university-industry interaction, education coordination, and policy support. By establishing joint research and development platforms, co-hosting research achievement exhibitions, and forming technology alliances, the mechanism promotes the transformation of scientific achievements. Through university visits to enterprises and co-establishment of graduate training bases, it advances industry-education integration. Special talent training programs and international talent classes are launched to strengthen high-level talent supply. A comprehensive political, economic, social, and technological (PEST)-based support framework is constructed to improve policy incentives and technical support systems. A representative party organization collaboration mechanism is established between Civil Engineering-focused Universities and Industry Chains, forming a replicable and scalable path for practice.