In response to the current disconnection between the teaching content of the structural testing course for postgraduate students in civil engineering and the demands of scientific research, the weakness of practical links, and the singleness of teaching resources, a teaching reform and practice of the structural testing course has been carried out. Based on research, discussions and teaching experience, the deficiencies existing in the current teaching of the structural testing course for postgraduate students have been summarized. The teaching objective of the structural testing course has been proposed as "facing the demands of scientific research + strengthening application ability". The teaching syllabus has been optimized and the teaching materials have been compiled based on the teaching content adjustment plan of "three increases and three decreases". A teaching method of "classroom + on-site", partially "teaching + learning" flipped classroom and "case + practical operation" integration has been constructed. An SPOC teaching platform combining online and offline and a case set of structural testing have been built. The teaching practice shows that the teaching reform has effectively improved the teaching quality of the structural testing course and the students" participation, and significantly enhanced the students" structural testing design ability and practical application ability.