Based on the university-enterprise cooperation idea, an innovative talent training mode in Wenzhou University was explored and practiced. With the help of university-enterprise cooperation platform, the resources of the enterprise can be fully used and the enterprise can take part in the whole progress of the talent training. In this paper, some cases of the talent training in civil engineering were fully introduced, such as the design of talent training plan, the innovation of the class teaching, the direction of the graduation project and professional practiceetc, which can provide references and lessons for other homologous local universities based on the university-enterprise cooperation.