1350 college stuedents were surveyed from five colleges in Chongqing province by questionnaires, and some results were achieved. The positive emotion experience of cooperative prosocial behavior in college students includes joyful emotion, progressive emotion and achievement emotion. The differences of the positive emotion experience of cooperative prosocial behavior are distinct between college students from different genders, city and country, singleton and non-singleton, and majors. There are significant or extremely significant correlations between college students’ cooperative prosocial behavior and joyful emotion or progressive emotion. The cooperative prosocial behavior types of public welfare and daily life have an important influence on the positive emotion experience. In order to improve the effectiveness, pertinence, diversity and sustainability of cultivation work in college students’ cooperative prosocial behavior, it is suggested that the strengthening of the positive emotion experience of cooperative prosocial behavior in college students should start from non-implemented stages and implemented stages mutually by various means and ways.