The frequent turnover behavior of second-generation of migrant workers leads to an increase in labor costs for enterprises, and hinders the process of migrant workers' urban integration. Based on the organizational support theory and reciprocity theory, this paper empirically tests the relationship and mechanism between perceived organizational support, sense of urban integration, reciprocity and turnover intention of second-generation of migrant workers by method of questionnaire survey. The results show that, perceived organizational support has a significant negative effect on turnover intention of migrant workers, and the sense of urban integration plays a mediating role. Moreover, the reciprocity tendency of migrant workers has a strengthening moderating effect on the relationship between sense of urban integration and turnover intention, and further moderates the mediating effect of sense of urban integration between perceived organizational support and turnover intention. In other words, the higher the reciprocity level of second-generation of migrant workers, the stronger mediating effect of sense of urban integration. This paper measures second-generation of migrant workers' reciprocity tendency for the first time and verifies that the micro enterprises' organizational support will promote second-generation of migrant workers integrate into the city. Therefore, the conclusion provides a theoretical guidance for the employer to better understand the psychological-behavior mechanism of second-generation of migrant workers and establish an effective management measures, at the same time, provides a gist for the realization path of second-generation of migrant workers integrate into the city.