To investigate the present situation of health risk behaviors, an anonymous questionnaire surveying on health risk behaviors was conducted among 1228 undergraduates from five universities in Chongqing. Results as follows：Health risk behaviors were common among the undergraduates. The prevalence of no seatbelt use was 60.6%.The prevalence of usual cigarette smoking and usual alcohol use was 14.7 % and 55.1 %. The prevalence of the net surfing and being addicted in net was 91.8% and 7.4%. The prevalence of feeling depressed was 45.1% and seriously considering of suicide attempt was 5.1%. 6.2% reported that they had the experience of sexual intercourse. The scores were significant difference in gender, grade, urban and rural areas, single child and living on campus. The male students who have poor academic performance, higher level of his father’s literacy and living off campus were more likely to develop health risk behaviors. There was a significant positive correlation between any two kinds of health risk behaviors of unintentional injury behavior, violence behavior, smoking, suicide, insecure sexual behavior and internet addiction disorder behavior.