Promoting the normalization, systematization and long-term effect of ecological civilization education is the key content of ecological civilization construction in the new era. It is also an important breakthrough for carrying out the basic task of moral cultivation in education, accelerating the construction of "double first class", and promoting the all-round development of students' morality, intelligence, physical education, beauty and labor. Ecological civilization education in universities is different from public ecological civilization education, and the education in universities has special internal multi-dimensional influence paths. On the basis of the current situation and influence mechanism of ecological civilization education in universities, the study constructs a multi-dimensional influence logical path model of ecological civilization education in universities, analyzes the internal mechanism that affects the level of ecological civilization education in universities, and does empirically test based on 1,239 questionnaires collected in universities in Tianjin. The conclusions show that the attention paid to ecological civilization is positively related to the systematization of ecological civilization education; the integrity of the teaching system is positively related to the regularization of ecological civilization education; situational factors will stimulate or hinder the long-term effect of ecological civilization education in universities; the regularization, systematization and long-term effect of ecological civilization education is positively related to the practice of ecological civilization behavior of students. The study proposes effective ways to improve ecological civilization education in universities.