This paper presents the experimental results of convective heat transfer performance in 5 copper tubes with three dimensional internally extended surface, with flow of various test fluid in the Reynolds number range of 8,000 to 80,000. Water and mixture of ethylene glycol with water (the ethylene glycol weight content equals 55%) are chosen as test fluid. The effect of the physical properties for test fluid on heat transfer performance in the tubes with three dimensional internally extended surface is discussed. The heat transfer correlation is obtained by the experimental data.