The errors of each parts of virtual instrument system, which are produced in the signals transfer process, are introduced. These errors include sensor error, signal conditioning error, data acquisition error and data dealing error, etc. The main error can be found by analyzing the influence of these errors on different virtual instrument system. The methods of errors modification are also discussed. It's an effective method that modifying system errors, especially nonlinear errors, by software. At last, as a good example, the error analysis and modification of Virtual Noise Analysis Instrument is given.