By analysis of electricallyevoked surface electromyogram(SEMG) to suralmuscle, study the characteristics of SEMG signal during muscle fatigue. The time stretchingof two semi-ave of M-wave are analyzed respectively by wavelet method choosing wavelet function matching to M-wave. Indexes of muscle fatigue are educed accordingto the scale of wavelet transform, are the quantitative analysis is realized during muscle fatigue. This method overcomes the limitation of Fourier Transform that it has no resolution in time domain, find indicators of SEMG which are correct, reliable and easy to interpret, and try to providetheoretic referencefor the applicationsof these indicators.