Differential scanning calorimetry(DSC) is a heat analysis method which studies matters' thermodynamic properties according the measured energy differential by temperature scanning under the control of linear temperature programme.Differential scanning calorimetry technique is used for the temperature and latent heat determination when some tissues in vitro such as pig muscle,fat,liver and beaf chang phase during the temperature rises.Then the latent heat and phase change temperature are derived.It is conclusion that the main influencing factors of latent heat and phase change temperature are water and fattiness in tissues;the more of water and the fewer of fattiness,the larger of latent heat and the boarder of the temperature area changing phase.