Masonry buildings with R/C frame- shear wall in the bottom storey have been widely used in city construction, which include two kinds of lateral force resistant system. However, the seismic behavior of the building is poor. Based on the characteristics that the rigidity of the upper masonry structure is much bigger than the rigidity of laminated rubber bearings and frame structure. It can be referred to as system having a two degree of freedom. The dynamic behavior of this building is analyzed, the isolated vibration mode controls the response of system. The authors give apractical method for dynamical analysis to masonry building with frame structure in the first storey with laminated rubber bearings between the first and second storey , which submits efficient path to found applicable design means and to generalize interlayer seismic isolation structure.