Q943.2
重庆市科技攻关项目
以杂交狼尾草叶片为材料,用0.05 mol NaOH溶液在沸水浴中加热处理10 min,进而用pH3.0的TE溶液进行中和获得DNA提取液,并用该DNA提取液为模板进行RAPD-PCR和SSR-PCR扩增.结果显示:用该方法提取的DNA与常规CTAB法提取的DNA质量相当,可用于以PCR技术为基础的DNA分子标记分析与96孔板技术相结合,可以实现DNA高通量提取.
A rapid method for DNA extraction from Pennisetum hybrid was developed.Using the method,leaves of the Pennisetum hybrid was treated by the 0.05 mol NaOH solution and heated in the boiling water for 10 min,followed by the neutralization with TE solution(ph=3.0),and the DNA extraction was used for RAPD-PCR and SSR-PCR analysis.The result indicates.The quality of the DNA by the simplified method is amount to DNA extracted by the CTAB method,and the DNA extracted by this method an meet the requirement of molecular marker analysis based on PCR technology.Combined with the 96-well plate,it can realize the high-throughput DNA extraction.
王凭青,吴明生,刘博,唐芳,李志中.杂交狼尾草分子标记分析DNA快速提取方法[J].重庆大学学报,2007,30(1):126-128.
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