以配位作用毛细管电泳为手段，采用非衍生直接紫外吸收检测，对板蓝根注射液中各组分进行分析。考察运行缓冲液的浓度和酸度、运行电压、进样时间以及毛细管内径等因素对分离和检测的影响。在优化条件下，即运行缓冲液为30 mmol/L CuSO4 水溶液，pH=3.26, 分离电压22 kV, 位差进样40 s, 毛细管内径50.0 μm，检测波长254 nm, 对其中含量最多的2种氨基酸——精氨酸和脯氨酸进行定量分析。分别考察其线性范围、检测限以及重现性。结果测得板蓝根注射液中精氨酸的含量为0.63 mmol/

The separation of primary amino acids in Radix Isatidis and its injection is performed by coordination interaction capillary electrophoresis with nonderivative direct UV detection. This study investigates the effect of concentration of Cu2+ and acidity of electrophoretic running buffer, separation voltage, injection time and the internal diameter of the capillary on separation and detection. Under the optimal operation condition, the two amino acids are qualitatively and quantitatively analyzed and its liner range, detection limit and reproducibility are investigated respectively. The results show that the content of arginine is 0.63 mmol/L and the content of proline is 25.7 mmol/L in the Radix Isatidis Injection. Under the same condition, the components of Radix Isatidis extracts are separated by the presented method. The mass fraction of arginine is 0.027 and the mass fraction of proline is 0.054.

国家自然科学基金资助项目(20775096)