For the comprehensive requirements of being dynamic，flexible，loosely coupled，and transparent of heterogeneous system integration in manufacturing enterprises，the conception of semantic gateway is proposed，and a new integration framework of heterogeneous system with the support of semantic gateway is established，to solve the semantic heterogeneous problem of heterogeneous systems and integrate the business flow of enterprises dynamically. On the basis，the semantic gateway model is established and semantic mapping standard，database adapter and service adapter of semantic gateway are deeply researched. Finally，the proposed studies are applied to the integration of heterogeneous information systems in a turbo & charger machinery manufacturing enterprise in Chongqing，and the application result is satisfying.