Nitrogen transformation characteristic experiments of organic fraction of municipal solid waste (OFMSW) in a batch hydrolysis-UASB two-phase anaerobic digestion are carried out to solve the problems of total ammonia-nitrogen (TAN) accumulation and obtain appropriate key process control parameters. Based on the experiment results, TAN accumulation prediction model in the two-phase anaerobic digestion of OFMSW with digestive effluent reuse is established. And then with the prediction model analyzed, reflux ratio is obtained as a key process control parameter to prevent TAN accumulation. In the end, verification tests are designed to validate the feasibility and practicability of resolving TAN accumulation by controlling reflux ratio. In verifying experiment, when reflux ratio is set at 0.85, TAN concentration of UASB effluent is increased to a constant value, which is 1 900 mg/L. Furthermore, the accumulated TAN has no negative influence on the population and activity of methanogens, on the contrary, it develops the buffer capacity to acid and alkaline and keeps the anaerobic system more steadily. To sum up, a method of resolving the problem of TAN accumulation by controlling reflux ratio of digestive effluent has been ascertained radically, and the method can be used in the preventing of ammonia inhibition in two-phase anaerobic digestion process.