The rehabilitation project of road tunnel in highland area and cold region is an important and worthy exploring research field. The development of the defects of crack in the liner, water leakage, structural deterioration and frost defects for Dabanshan road tunnel are studied. After an insight into the cause and development of tunnel defects, the water leakage and frost damage are considered as the root of defect, so restoration of drainage system and the reinstallation of cold prevention system are the focuses of the rehabilitation project. For consideration of safety, cover arch is installed for the tunnel structure enforcement as well. Results indicate measures in Dabanshan road tunnel rehabilitation project are effective for road tunnel of high altitude and tunnel in cold region.