The purpose of this paper is to identify the effects of toluene and the toluene content which is at different initial temperatures on the ignition delay time of diesel surrogate fuel under homogeneous charge compression ignition (HCCI) engine condition. The CHEMKIN4.1 module (closed homogeneous batch reactor) is used with the constructed diesel surrogate fuel chemical kinetics model to simulate the constant volume. The results show that the ignition property is mainly characterized by n-heptane. The presence of the slowly oxidizing toluene in the surrogate fuel does not affect the auto-ignition time considerably. In addition,the influence of toluene content on the ignition delay time is significant only at low initial temperature. More ignition delayed combustion processes have been found when toluene content is increasing.