The effect of mixed sulphur bacilli on the bioleaching of chalcopyrite tailings in shake flask and the effect of different citric acid concentrations and slurry densities on leaching tailings are studied. The results show that the concentration of citric acid in a certain range can promote chalcopyrite tailings bioleaching, and the highest of leaching rate is 44.8%. The citric acid concentration and slurry density directly affect the oxidation activity of bacteria and the effect of leaching.