Experiments are carried out on high-phosphorus oolitic hematite in western Hubei, using the method of reduction roasting and low intensity magnetic separation, and Aspergillus niger is used to do the research of bioleaching dephosphorization on concentrate.The orthogonal test results show that the descending order of factors influencing concentrate grade is calcinations temperature, roasting time, reduction ratio, grinding grain.The best condition for reduction roasting and low intensity magnetic separation is calcinations temperature 900 ℃, roasting time 25 min, reduction ratio 6%, and grinding grain -0.074mm95.08%.On these conditions, concentrate grade is 57.25% and recycling rate is 90.20%.Aspergillus niger has a strong removal capability on phosphorus in concentrates.After 8 days of microbiological leaching, removal of phosphorus is 79.68% with lower pulp density, and the percentage of phosphorus is reduced to 0.17% from 0.85%, which provides the foundation of application on dephosphorization by microorganism.