The immobilized TiO2 film loaded on fiberglass mesh is prepared by sol-gel method, and the analysis result of X-ray diffraction (XRD) shows that the crystalline phase is anatase. On the basis of UV/TiO2 system, the degradation results and effect factors of algal intracellular organic matter(IOM) in Tai Lake are studied. The results show that the degradation of IOM is much harder by photocatalytic oxidation in comparison with humic acid(HA). In 60 min, the IOM degradation of UV254 and DOC are 33.3% and 19.1%, but the HA solution are 96.6% and 57.3% respectively. The reason lies in that IOM is mainly comprised of macromolecular hydrophilic organic matter, containing more amino structures, while HA is strongly hydrophobic organic matter, containing more aromatic structures. Meanwhile, the degradation of IOM is improving as the increase of light intensity to a certain extent; the best degradation rate of IOM appears at pH=6.7.