To realize automatic control, it is important to study the response of reactor to electric field. A three-dimensional biofilm eletrode reactor is developed and steadily operated to remove nitrogen. In the reactor, oxygen is produced on the anode and hydrogen is produced on the cathode of the three-dimensional electrode by electrolysis water. Microorganism in the reactor utilize oxygen and hydrogen adequately to remove nitrogen by nitrification-denitrification. To evaluate the correlation of electric field with the reactor, DO, pH and nitrogen removal efficiency are studied. Results show that when a electric field of 0.013 4 mA/cm 2 is applied to the system, the removal rate of NH +4-N, NO -3-N and TN is 90%, 70% and 70%, respectively. To assure the reactor run efficiently, the maximum of the intensity of electric field applied to the reactor is 0.0201 mA/cm 2. Within 0.0201 mA/cm 2, the system is in stable running status, while DO and pH is altered resulting from the intensity of electric field. With enhancing the intensity of electric field, the removal rate of NO -3 -N can be improved, although, the removal rate of NH +4-N is not elevated markedly. There is no accumulation of NO -2 -N within 0.0201 mA/cm 2.