Microbial pretreatment can enhance biogas production from corn stover through solid-state anaerobic digestion, but the price of microbial strains is high. Stack-pretreatment is introduced to gain higher biogas production from corn stover through solid-state anaerobic digestion. The purpose is to find the effects of naturally microbial pretreatment on stalk lignin, cellulose and hemicellulose degradation, and biogas production. The result indicates that the corn straws which has been stack-pretreated for 20 days decreases of the content of the middle corn straws cellulose, hemi-cellulose and lignin by 5.8%, 16.8% and 5.7% respectively lower than those of untreated samples. The highest cumulative biogas yield for 60-day digestion is obtained in the group that the mass ratio of corn stover and cattle dung is 1∶1, which is 24.4% higher than that of untreated corn stover mixed with cattle dung with the same mass ratio. When the mass ratio of corn stover and slug is 1∶1, the cumulative biogas is 23.27% higher than that of untreated corn stover with slug.