The effects of cold rolling process and interannealing on the microstructure and texture evolution of commercial pure titanium sheets are studied by advanced electron backscatter diffraction (EBSD) technique. Through comparing the EBSD orientation distribution, orientation difference angle distribution and pole diagram of samples with different primary cold rolling deformation after annealing and secondary cold rolling, it is known that the twins produced by first cold rolling process have an important effect on the grain size and orientation (texture) of annealed recrystallized grains, which proceeds to affect the deformation structure and texture characteristics of secondary cold rolling, and the formation of deformation twins in secondary cold rolling is restrained to a certain extent. The twin fraction increases first and then decreases with the increasement of rolling deformation. At the same time, the {0001} basal texture component in secondary cold rolling decreases.