The non-visual biological effect of light is closely related to human health. With the wide application of LED in indoor lighting, great attention has been paid to the relationship between LED lighting and human physiological health. Electrocardiograph (ECG) is one of the main characteristics of physiological parameters. Therefore, the response of ECG indicators of the elderly to LED lighting in different light exposure duration (10 min, 20 min) were investigated by taking three levels of color temperature (3000 K, 5000 K and 6500 K) and five levels of illuminance (300 lx, 500 lx, 750 lx, 1 000 lx and 1500 lx) as main variables. Experimental results indicate that ECG indicators of the elderly are not affected by main effects of color temperature and illuminance, but indeed affected by the interaction of illuminance and color temperature, wherein P wave time and T wave time are most affected by the interaction of 3 000 K and 1 000 1x, and least affected by the interaction of 6500 K and 1 500 1x; PR interval is most affected by the interaction of 5 000 K and 750 lx, and least affected by the interaction of 6 500 K and 500 1x. However, the changes of ECG of the elderly are not significantly influenced by the short light exposure duration, so it is necessary to further explore the response of ECG indicators of the elderly under long-term (1 h or more) exposure so as to provide sufficient basis for the determination of healthy lighting parameters for the aged.