A two dimension opto electronic test method has been developed to determine the distribution characteristics of cancellous bone porosity. The fresh pig tibiae concellous bone in behind leg was sectioned. A total of 36 cancellous bone sections were divided two groups. The first group consisted of 18 transversal sections along the axial direction, and the second group consisted of 18 axial sections along the transversal direction of the tibiae. Their images were examined microscopically and processed quantitatively. The total area, solid matrix area and pore area of each specimen and its porosity were determined, too. The cancellous bone porosity is gradually decreased along the axial direction of tibiae and approaching to the compact bone, but almost constant along the transversal direction. Cancellous bone is modeled as a transversely isotropic, liquid saturated porous solid. The two dimension opto electronic test method can be used to relatively accurately determine the distribution characteristics of the cancellous bone porosity from different directions if the cancellous bone sections are enough.