Photoacoustic spectroscopy (PAS) is a highly sensitive gas analyzing method without the shortcoming of gas consumption, which can be used in transformer oil trace gases dissolved in on line monitoring. The photoacoustic signal is detected with a microphone, which is the only feature in gas concentration analysis. A portable and tunable experimental setup has been developed based on the photoacoustic spectroscopy fundamental. Through the photoacoustic signal stimulate mechanism analysis, this paper thoroughly discusses the influences of gas pressure, temperature, laser power, gas concentration, background gas, chopped frequency and the resonant frequency drift on the gas photoacoustic signal. The theoretical and experimental results supply reference and technical support for further improvements in oil gas photoacoustic spectroscopy of on-line monitoring system.