Dynamic performance test methods and experiments of motorcycle engine are investigated. The models of clutch, transmission and brake are simulated by changing the resistance torque imposed by the dynamometer to the engine and with the moment of inertia and road resistance data, the model of a 125 mL motorcycle is established on the dynamic engine test bench. On this basis, the road tests are simulated with models of different inertial mass. Compared with the experiments on the drum tester, the results show that when the inertial mass is set to 1.1 times the datum mass of the motorcycle, the deviations of its maximum speed, sliding distance and acceleration time are no more than 3% and the fuel consumption of 100 km is no more than 5% except the speed of 35 km/h. Through driver operational model, the effect of different gear change models on vehicle acceleration performance is studied. Finally, the regulatory driving cycle for motorcycle is implemented on the dynamic bench and the measurements show that it has high ability to accurately control speed.