The organic-inorganic hybrid catalyst,namely[GlyH]_xH_3-xPW₁₂O₄₀(x=1.0-3.0),was prepared by combining phosphotungstic acid and glycine (Gly).The effects of various factors on the catalytic synthesis of isoamyl acetate with using[GlyH]_xH_3-xPW₁₂O₄₀(x=1.0-3.0) as catalysts were studied,and the synthesis process of isoamyl acetate was optimized by response surface methodology (RSM).The results show that[GlyH]_1.0-H_2.0PW₁₂O₄₀ catalyst has the highest catalytic esterification reactivity and reusability.The optimum conditions are n(isoamylol):n(acetic acid)=1.05:1,the amount of catalyst to acetic acid 4.5%,reaction time 2.0 h and water-carrying agent 10 mL.Under these conditions,the yield of isoamyl acetate reaches 98.5%,which is in basic agreement with values predicted by the mathematical model.Under the optimal conditions,the active energy of the esterification is 79.73 kJ/mol.