Building module with synthetic biology to change the genetic background of E.coli and produce the 4(5),11(12)-Taxadiene that originally comes for Taxus chinensis as a secondary metabolite. First, MEP2(2-C-methyl-D-erythritol-4-phosphate) module is built and it includes four inherent enzymes, i.e. ispC, ispE, ispH and ispG. The four genes all belong to the MEP upstream pathway. Then, the MEP2 module is transferred into E.coli which contains the upstream MEP1 and downstream TC by electroporation. Finally, these gene expresses are induced with using IPTG and the products are detected by GC-MS. The MEP2 gene module is built successfully and MEP2 is convinced to enhance the production of taxadiene through several combination experiments continually. E.coli can be rebuilt to produce the botanical drug taxol precursor taxadiene and other intermediates by genic engineering.