In order to conduct demonstration teaching of reinforced concrete structure experiment, the research and development of experiment teaching demonstration system based on virtual instrument have been done independently. With NI cDAQ the mixed-signal acquisition system is built, the signal acquisition and data processing software is designed for experiment demonstration teaching. The functions of data clearing, data correction, data recording and drawing are achieved. Practice result shows that the demonstration system can lay a solid foundation for the stereoscopic heuristic experiment teaching and interactive experiment teaching. With the new test technology applied to teaching, the teaching efficiency and quality are increased. The experiment teaching demonstration system has a good application value.