The Rural Revitalization and the construction of Guangdong-Hong Kong-Marco Greater Bay Area provide new development opportunities for civil engineering talent training in agricultural universities. In order to realize the goal of integrative talents training of civil engineering in agricultural universities, which is "mastering the knowledge of management, design and construction", taking South China Agricultural University as an example, a "five-in-one" talent training mode reflecting characteristics of agricultural universities is constructed by integrating teaching resources inside and outside school, and following the school-running characteristics of "industry-based, combination of industry and agriculture". The curriculum system, experimental teaching system, science and technology team of students, teaching staff, teaching quality monitoring and guarantee system have been reformed. Practice shows that the "five-in-one" talent training model has achieved good results in terms of teaching conditions, teaching and research level of teachers, students' comprehensive ability, and social impact.