The international accessible design theories of architecture have evolved into humanized design trends such as universal design. Some universities in China have also established accessible design teaching systems. Experimental teaching in professional courses can make students deeply understand the importance of accessible design, but the traditional accessible simulation experiment can not meet the requirements of students to complete the experiment concurrently, carry out comprehensive design practice learning, and adapt to the requirements of course construction. The virtual simulation experiment platform for accessible universal sign environment design refers to the teaching model concept of evidence-based design, combines basic principles with practical operation experience, and guides students to deepen learning according to levels through six systems and three levels. The introduction of virtual simulation experiment extends the traditional teaching and achieves the innovation of teaching methods and teaching evaluation system. From the feedback of classroom teaching and experimental reports, virtual simulation experiment for accessible design teaching can improve students' professional quality, stimulate students' innovation consciousness, enhance students’ comprehensive ability and cultivate students' social responsibility, which is an effective way to promote the upgrade of accessible design and architecture education.