Many large and medium cities in China are entering the era of rail transit, yet new science popularization channels has not been developed and the quality of science popularization works has not been improved. Science popularization is lagging behind the rapid development of rail transit construction in China. However, “micro learning resources” meets the learners’ individualized needs, and is suitable for science popularization knowledge communication in the mobile learning era. In view of the current situation that popular science popularization is lagging behind in the rapid development of rail transit, popular science demand and key characteristics of “micro learning resources” are analyzed from the matching perspective to investigate the matching status and problems. On the basis of partial adjustment of the key features of the “micro learning resources”, the perfect matching “Science Popularization - Micro learning resources” system of rail transit is put forward. Taking WeChat public number as the carrier of science popularization, the teaching resources of “micro learning resources” are used to carry out knowledge popularization of rail transit among urban residents and improve the effect of science popularization.