The key laboratory of the ministry of education is an important Public Platform for scientific research experiments in universities. Take the Key Laboratory of New Technology for Construction of Cities in Mountain Area for example, combined with practical problems, this paper introduces five main experiences in laboratory construction : Rationalization of talent echelon construction by introducing leading talents; Enhance research security by increasing hardware investment; Integration of industry, education, research and application to promote the development of science and education platform; Based on the characteristics of mountainous areas, expanding academic exchanges with foreign countries; Managing virtual and reality alternation, realizing hardware environment usage to be shared. It’s helpful for operation and construction management of the key laboratory of Education Ministry.