n the mobile Internet era, online and offline mixed teaching mode has become a new information-based teaching mode vigorously promoted by universities. With the purpose of exploring the application effect of online and offline mixed teaching mode in university and thus effectively improving students’ professional knowledge level, taking undergraduate students who major in the course of engineering economics A of Civil Engineering College of Southeast University as the research object. It takes MOOC online teaching platform as the data source, uses SPSS24.0 software for statistical analysis, and analyzes the online and offline learning situation of undergraduate students from four indicators: the number and duration of video viewing, attendance and the accuracy rate of classroom practice. Finally, the final examination results are analyzed from two aspects of online performance and after-class homework performance. The results show that there is a significant positive correlation between online scores, after-class homework scores and final examination scores. Online and offline mixed teaching mode is beneficial to develop learners’ scientific literacy of autonomous learning and inquiry practice, which can improve learning effect.