The space is the carrier of ecological civilization construction and the priority of ecological civilization construction is the core value of the construction of territorial space planning system. In the process of integrating urban and rural planning into territorial space planning, the concept, focus and technical methods of planning preparation have also undergone corresponding changes. In order to better implement the idea of ecological civilization in the teaching of national space master planning course for undergraduates majoring in urban and rural planning; At the same time, in order to solve the key problems and difficulties in the transformation of current teaching in response to the development requirements of the new era, many architecture colleges have been actively exploring and practicing. This paper takes the teaching of the master planning of land and space of the fourth grade undergraduate of Kunming University of Science and Technology as an example to explore the specific measures of teaching reform, including the guidance of ecological civilization as the core value, the expansion of the knowledge structure of the intersection of ecology and urban and rural planning, and the strengthening of the application of GIS and other technologies. Finally, the teaching reform practice was demonstrated from three aspects: "evaluation of the carrying capacity of resources and environment and the suitability evaluation of land and space development", the delimitation of three zones and three lines, and the blue-green space planning of the central urban area, with a view to providing a practical path to meet the teaching requirements of the overall land and space planning course of urban and rural planning specialty in the new era.