Strengthening the construction of Party branches is an important part of the great project of Party building in the new era. How to play the role of party branches and improve organizational efficiency has become a key issue in the construction of party branches. Under the background that the Party Central Committee puts forward higher and higher requirements for scientific and technological power, the construction of university party branch is particularly important. By discussing the influencing factors of party spirit cultivation of Party members, analyzing the relationship between Party spirit and human nature, the shaping of collective culture by production relations, the influence of the concept of "karma, good karma" in traditional culture and positive feedback incentive mechanism on human behavior, and the interpersonal characteristics and working characteristics of college teachers. To construct a positive incentive mechanism with interest guidance and positive incentives as means, and to shape a "positive feedback loop" micro-environment, to improve party spirit cultivation of party members as the goal, to solve the problems of improving organizational efficiency, and put forward the feasible way to strengthen the branch construction and realize the continuous optimization of university micro-governance.