To meet the needs of high speed development of current economic society, the practice teaching of application oriented colleges must be constructed rationally to realize the goal of vocational ability training. According to the theme, the reform measures for the practice teaching of civil engineering specialty in application oriented colleges were researched and discussed. Based on the analysis of current situation and existing problems of practice teaching, a new practice teaching mode of “a thought, three main lines, and four levels” was constructed according to characteristics of civil engineering. The new practice teaching mode was operated in the practical teaching process. The result shows that by using the new practice teaching mode, undergraduates’ professional consciousness became strong and their vocational abilities were recognized by employing units.