Combined with the characteristic training mode of the cooperative education of working and learning, we reformed on the application oriented talent training mode from aspects of training objectives, training plans, theory course systems and the practical course system. Based on the social demand for professional talent, we developed a new training program and plan, optimized the professional curriculum system, and constructed the practice course system of three modules (experiment, practice, and design) and three levels (foundation, comprehensive, and innovation). Achievements were made in aspects of practice base construction, textbook construction, teaching staff construction, and students' innovation ability cultivation. Finally, a new practice teaching mode "experience construction" was presented.