Based on the case of Xuzhou Institute of Technology which is a newly established undergraduate college，firstly the adaptability of cultivating innovative and applied talents of civil engineering in such a college was analyzed. Then, its orientation and general requirements of innovative and application-oriented talent training were proposed. At last, combined with the actual situation in this school, from the four aspects such as the course construction and reform, practice teaching base and platform construction, teaching way and method reform and teacher team construction, the exploration and practice of training innovative and application-oriented talents in civil engineering specialty were detailed in order to provide a reference model of cultivating talents for the similar newly established undergraduate colleges.